Title:

Prodrugs activated by plasmin and their use in cancer chemotherapy

Document Type and Number:

United States Patent 7402556

Abstract:

The product of the invention is a modified form of a therapeutic agent and comprises a therapeutic agent, an oligopeptide having a plasmin peptide substrate of 2-4 amino acids and mono- or di-peptide linkage, a stabilizing group and, optionally, a linker group. The prodrug is cleavable by plasmin. Also disclosed are methods of making and using the prodrug compounds.

Inventors:

Trouet, André (Herent, BE)
Dubois, Vincent (Fleurus, BE)
Passioukov, Alexandre (Brussels, BE)

Plaque It!

TECHNICAL FIELD

The present invention relates to compounds useful as prodrugs that are activated by tumor-secreted enzymes. More particularly, the invention relates to prodrugs having a novel mono- or di-peptide linkage and a plasmin peptide substrate of 2-4 amino acids. Such prodrugs may be used for treatment of disease, especially in cancer chemotherapy.

BACKGROUND

Many therapeutic agents, such as anthracyclines and vinca alkaloids, are especially effective in cancer chemotherapy. However, these agents often exhibit acute toxicity iii vivo, especially bone marrow and mucosal toxicity, as well as a chronic cardiac toxicity in the case of the anthracyclines and chronic neurological toxicity in the case of the vinca alkaloids. Similarly, methotrexate may be used for the treatment of inflammatory reactions, such as rheumatic diseases, but its high toxicity limits its applications. Development of more and safer specific antitumor agents is desirable for greater effectiveness against tumor cells and a decrease in the number and severity of the side effects of these products (toxicity, destruction of non-tumor cells, etc.). Development of more specific anti-inflammatory agents is also desirable.

The search for more selective anticancer agents has been extremely active for many decades, the dose limiting toxicities (i.e. the undesirable activity of the anticancer agents on normal tissues) being one of the major causes of failures in cancer therapy. Accordingly, the goal has been to improve the specificity of anti-tumor agents for increased effectiveness against tumor cells, while decreasing adverse side effects, such as toxicity and the destruction of non-tumor cells.

The focus of research has been on the development of new therapeutic agents which are in the form of prodrugs, compounds that are capable of being converted to drugs (active therapeutic compounds) in vivo by certain chemical or enzymatic modifications of their structure. For purposes of reducing toxicity, this conversion is preferably confined to the site of action or target tissue rather than the circulatory system or non-target tissue. Prodrugs are often characterized by a low stability in blood and serum, due to the presence of enzymes that degrade or activate the prodrugs before the prodrugs reach the desired sites within the patient's body. A desirable class of prodrugs that addresses such problems has been disclosed in Trouet, et al., U.S. Pat. No. 5,962,216 and in Lobl, et al., PCT International Publication No. WO 00/33888, both of which are incorporated herein by reference.

Tripeptide derivatives of anticancer agents such as daunorubicin (“DNR”) and doxorubicin (“DOX”), to be used as prodrugs were studied by Chakravarty, et al., J. Med. Chem. 26:633-638, 1983 (A); Chakravarty, et al., J. Med. Chem. 26:638-644, 1983 (B); and Balajthy, et al., J. Med. Chem. 35:3344-3349, 1992. However, none of these approaches has been shown to be successful.

Other work in this area includes Monsigny, et al., FEBS Letters 119(1):181-186, 1980 (DNR); Baurain, et al., U.S. Pat. No. 4,296,105 (DNR); Baurain, et al., J. Med. Chem. 23:1171-1174, 1980 (DNR); Masquelier, et al., J. Med. Chem. 23:1166-1170, 1980 (DNR); and de Groot, et al., J. Med. Chem. 42:5277-5283, 1999 (DNR and DOX), which all describe prodrugs comprising a carrier linked to the drug via a peptide arm. Typically, these references describe a peptide arm, linked via its free carboxyl function to the free amine function of derivatives of anthracyclines such as DNR. In addition, the arm of these prodrugs can be linked via its free amine function to a carrier consisting of a macromolecule (protein such as BSA, immunoglobulins, etc.) which permits the selective endocytosis of the prodrug by target cells.

However, in spite of the advances in the art, there continues to be a need for the development of useful prodrug compounds and methods of making such prodrugs. Prodrugs that display a high specificity of action, reduced toxicity, and improved stability in blood relative to known prodrugs of similar structure are particularly desirable. The instant invention addresses those needs.

SUMMARY OF THE INVENTION

One aspect of the invention relates to compounds that are prodrug forms of therapeutic agents with a mono- or di-peptide linkage and a plasmin peptide substrate of 2-4 amino acids (i.e., a dipeptide, tripeptide or tetrapeptide substrate), which in turn, is linked to a stabilizing group. In essence, the prodrug comprises a plasmin-recognizable peptidic sequence linked to one or two aliphatic amino acids having a large lateral chain which aliphatic amino acid is further linked to the drug. These prodrugs display a high specificity of action, reduced toxicity, improved stability in the serum and blood, and exhibit minimal movement into target cells unless activated by a target cell associated enzyme.

In one aspect of the invention, the compound comprises: (1) a therapeutic agent capable of entering a target cell; (2) an oligopeptide having the formula X-Y, where X is a plasmin peptide substrate of 2-4 amino acids and Y is a peptide comprising 1-2 aliphatic amino acids having large lateral chains; (3) a stabilizing group; and (4) optionally, a linker group not cleavable by plasmin; wherein the oligopeptide is directly linked to the stabilizing group at a first attachment site of the oligopeptide and the oligopeptide is directly linked to the therapeutic agent or indirectly linked through the linker group to the therapeutic agent at a second attachment site of the oligopeptide; wherein the stabilizing group hinders cleavage of the oligopeptide by enzymes present in whole blood; and wherein the compound is cleavable by plasmin. In anther aspect of the invention, the compound is selectively cleavable by plasmin.

In another aspect of the invention, the oligopeptide has the formula (AA ^x) _m-(AA ^y) _n(SEQ ID NO:1) wherein: (AA ^x) _mis a plasmin substrate and each AA ^xindependently represents an amino acid; each AA ^yindependently represents an aliphatic amino acid having a large lateral chain; m is an integer from 2-4; and n is an integer from 1-2, said oligopeptide being cleavable by plasmin.

Another aspect of the invention pertains to a pharmaceutical composition comprising these prodrugs and optionally a pharmaceutically acceptable carrier.

Yet another aspect of the invention pertains to compounds comprising a marker useful in the characterization of tumors, for example, diagnosis, progression of the tumor, and assay of the factors secreted by tumor cells. Another aspect of the invention relates to articles of manufacture for diagnosis or conducting assays comprising: (1) a compound comprising (a) a marker, (b) the oligopeptide described above, (c) a stabilizing group, and (d) optionally, a linker group not cleavable by plasmin; and (2) at least one reagent useful in the detection of the marker.

Another aspect of the invention relates to methods of treating a medical condition by administering a prodrug of the invention to a patient in a therapeutically effective amount.

Yet another aspect of the invention pertains to a method for decreasing the toxicity of a therapeutic agent wherein the therapeutic agent is intended for administration to a patient, the method comprising: covalently forming a prodrug by linking the oligopeptide described above to a stabilizing group at a first attachment site of the oligopeptide and directly or indirectly linking the therapeutic agent at a second attachment site of the oligopeptide, whereby the prodrug provides for decreased toxicity of the therapeutic agent when administered to the patient.

DETAILED DESCRIPTION OF THE INVENTION

The invention describes prodrugs that (1) are sufficiently stable in the bloodstream and in the biological fluids, to achieve important concentrations at tumor level in its non-hydrolyzed form; (2) are unable to enter, as such, into either normal or tumoral cells; and (3) are activated extracellularly by proteases secreted by tumor cells and having a hydrolysis rate considerably high to provide the liberation of sufficient quantities of active agent capable of entering cells and reaching its intracellular targets. The prodrugs are activated by plasmin, an enzyme overproduced in the vicinity of a large number of human tumor cells.

Before proceeding with the description of the invention, it may be helpful to set forth the abbreviations used herein.


Abbreviations


ACN	Acetonitrile
B16—B16	B16—B16 melanoma cells
BFS	Bovine fetal serum
Boc	t-Butyloxycarbonyl
BSA	Bovine serum albumin
Bz	Benzyl
Cbz	Benzyloxycarbonyl
CM	Conditioned medium
DBN	1,5 Diazabicyclo [4.3.0] non-5-ene
DBO	1,4 Diazabicyclo [2.2.2] octane
DBU	1,8-Diazabicyclo [5.4.0] undec-7-ene
DCC	N,N′Dicyclohexylcarbodiimide
DCM	Dichloromethane
DIC	N,N′-Diisopropylcarbodiimide
DIPEA	Diisopropylethylamine
DMEM	Dulbecco's Modified Eagle Medium
DMF	Dimethylformamide
DNR	Daunorubicin
DMSO	Dimethylsulfoxide
DOX	Doxorubicin
EDTA	Ethylenediaminetetraacetic acid, tetrasodium salt
EtOH	Ethanol
Et ₂O	Diethyl ether
Fmoc	9-Fluorenylmethyloxycarbonyl
HATU	O-(7-Azabenzotrazol-1-yl)-1,1,3,3-tetramethyluronium-
	hexafluorophosphate
HBTU	2-(1H-Benzotriazole-1-yl)1,1,3,3-tetramethyluronium-
	hexafluorophosphate
HOBT	N-Hydroxybenzotriazole
HPLC	High pressure liquid chromatography
KSCN	Potassium isothiocyanate
Me	Methyl
MeOH	Methanol
MeOSucc	Methyl hemisuccinate or methyl hemisuccinyl
NaOAc	Sodium acetate
NMP	N-methylpyrrolidone
NMR	Nuclear magnetic resonance
OSu	the N-hydroxysuccinimide ester
PAM resin	4-hydroxymethylphenylacetamidomethyl
PBS	Phosphate buffered saline: NaH ₂PO ₄2.8 mM, NaHPO ₄7.2
	mM, NaCl 150 mM, pH 7.4
PEG	Polyethylene glycol
PyBOP	benzotriazole-1-yl-oxy-tris-pyrrolidino-phosphonium
	hexafluorophosphate
Pyg	Pyroglutamic acid
RT	Room temperature
SPPS	Merrifield solid phase peptide synthesis method
Succ	Succinyl Acid/Succinyl
tBu	tert-Butyl
TCE	trichloroethyl
TEA	Triethylamine
TES buffer	Tris-HCl 50 mM, EDTA 50 mM in H ₂O, pH 8.0
TFA	Trifluroroacetic acid
THF	Tetrahydrofuran
TLC	Thin Layer Chromatography
Tos	p-Toluenesulfonyl

Prodrugs

The prodrug of the invention is a modified form of a therapeutic agent and comprises several portions, including: (1) a therapeutic agent; (2) an oligopeptide; (3) a stabilizing group; and (4) optionally, a linker group. Each of the portions of the prodrug are discussed in greater detail below. A typical orientation of a prodrug of the invention is as follows:

(stabilizing group)-(oligopeptide)-(optional linker group)-(therapeutic agent) The stabilizing group is directly linked to the oligopeptide at a first attachment site of the oligopeptide. The oligopeptide is directly or indirectly linked to the therapeutic agent at a second attachment site of the oligopeptide. If the oligopeptide and the therapeutic agent are indirectly linked, then a linker group is present.

As used herein, the term “direct” linkage of two portions of the prodrug means a covalent bond exists between the two portions. The stabilizing group and the oligopeptide are directly linked via a covalent chemical bond at a first attachment site of the oligopeptide. Similarly, the oligopeptide and the therapeutic agent can be directly linked by a covalent bond at a second attachment site of the oligopeptide.

As used herein, the term “indirect” linkage of two portions of the prodrug means each of the two portions is covalently bound to a linker group. Accordingly, in an alternative embodiment, the stabilizing group and the oligopeptide are directly linked via a covalent chemical bond at a first attachment site of the oligopeptide and the oligopeptide and the therapeutic agent are indirectly linked by a linker group at a second attachment site of the oligopeptide.

The first attachment site of the oligopeptide is typically at the N-terminus and the second attachment site is typically at the C-terminus. However, in an alternative embodiment, the orientation of the prodrug may be reversed so that the stabilizing group is directly linked to the C-terminus of the oligopeptide and the therapeutic agent is directly or indirectly linked to the N-terminus of the oligopeptide. Thus, in the alternative embodiment, the first attachment site may be the C-terminus of the oligopeptide and the second attachment site of the oligopeptide may be the N-terminus of the oligopeptide. The alternative embodiment of the invention functions in the same manner as does the primary embodiment.

The oligopeptide portion of the prodrug of the invention comprises two regions: a plasmin peptide substrate and a 1-2 aliphatic amino acid linkage. In order for the prodrug to be effective, the prodrug typically undergoes in vivo modification and an active portion, i.e., a transport-competent portion, of the prodrug enters the target cell. A first cleavage within the oligopeptide portion of the prodrug may leave an active or transport-competent portion of the prodrug as one of the cleavage products. Alternatively, further cleavage by one or more peptidases may be required to result in a portion of the prodrug that is capable of entering the cell. The active portion of the prodrug has at least the therapeutic agent and is that part of the prodrug that can enter the target cell to exert a therapeutic effect directly or upon further conversion within the target cell. Thus, the compound has an active portion, and the active portion is more capable of entering the target cell after cleavage by plasmin, an enzyme associated with the target cell, than prior to cleavage by plasmin.

The structures of the stabilizing group and oligopeptide are selected to limit clearance and metabolism of the prodrug by enzymes that may be present in blood or non-target tissue and are further selected to limit transport of the prodrug into the cells. The stabilizing group blocks degradation of the prodrug and may act in providing preferable charge or other physical characteristics of the prodrug. The amino acid sequence of the oligopeptide is designed to ensure specific cleavage by plasmin.

It is desirable to make a therapeutic agent, especially an antitumor and/or anti-inflammatory therapeutic agent, inactive by modification of the therapeutic agent to a prodrug form. According to the invention, the target cells are usually tumor cells or cells participating in inflammatory reactions, especially those associated with rheumatic diseases, such as macrophages, neutrophils, and monocytes. Modification of the therapeutic agent to a prodrug form also reduces some of the side effects of the therapeutic agents.

In the target cell, the therapeutic agent (optionally attached to one or two aliphatic amino acids and possibly also a linker group) acts either directly on its specific intracellular action site or, after a modification by intracellular proteases, kills the target cell or blocks its proliferation. Since normal cells do not activate plasminogen into plasmin in vivo as is seen with tumor cells, the prodrugs of the invention are maintained inactive and do not enter the normal cells or do so to a relatively minor extent.

The prodrug is administered to the patient, carried through the blood stream in a stable form, and when in the vicinity of a target cell, is acted upon by plasmin. Since the enzyme activity is only minimally present within the extracellular vicinity of normal cells, the prodrug is maintained and its active portion (including the therapeutic agent) gains entry into the normal cells only minimally. In the vicinity of tumor or other target cells, however, the presence of plasmin in the local environment allows for cleavage of the prodrug. Once the prodrug is cleaved extracellularly, the transport-competent or active portion gains entry into the target cell. Once within the target cell, it may be further modified to provide therapeutic effect, such as by killing the target cell or blocking its proliferation. While a portion of the prodrug may occasionally gain access to, and possibly harm normal cells, the transport-competent portion of the drag is freed primarily in the vicinity of target cells. Thus, toxicity to normal cells is minimized.

This process is particularly useful for, and is designed for, target cell destruction when the target tissue excretes an enzyme or other factor that is not secreted by normal cells. Here “normal cells” means non-target cells that would be encountered by the prodrug upon administration of the prodrug in the manner appropriate for its intended use. Since normal cells liberate little or none of the target-cell enzyme(s) that are responsible for cleaving the bond that links the active portion (including the therapeutic agent) of the prodrug from the remainder of the prodrug in vivo, the compound of the invention is maintained inactive and does not enter the normal cells.

As described in greater detail below, the prodrugs of the invention are compounds comprising:

(1) a therapeutic agent capable of entering a target cell;

(2) an oligopeptide having the formula X-Y, where X is a plasmin peptide substrate of 2-4 amino acids and Y is a peptide comprising 1-2 aliphatic amino acids having large lateral chains;

(3) a stabilizing group; and

(4) optionally, a linker group not cleavable by plasmin;

wherein the oligopeptide is directly linked to the stabilizing group at a first attachment site of the oligopeptide and the oligopeptide is directly linked to the therapeutic agent or indirectly linked through the linker group to the therapeutic agent at a second attachment site of the oligopeptide;

wherein the stabilizing group hinders cleavage of the oligopeptide by enzymes present in whole blood; and

wherein the compound is cleavable by plasmin.

As used herein, “cleavable by” means cleavable under physiological conditions.

The oligopeptide can also be described as: (AA ^x) _m-(AA ^y) _n(SEQ ID NO:1) wherein: (AA ^x) _mis a plasmin substrate and each AA ^xindependently represents an amino acid; each AA ^yindependently represents an aliphatic amino acid having a large lateral chain; m is an integer from 2-4; and n is an integer from 1-2.

Target Cell Associated Enzyme: Plasmin

The prodrugs of the invention are designed to take advantage of preferential activation through interaction with plasmin, an enzyme associated with the target cell, at or near the site targeted within the body of the patient. The involvement of proteases, such as the plasminogen activation system, in the cascade of events leading to the metastatic spread of cancer cells has been well documented. Based on the involvement of this system in malignancy, and on the concept and technology previously developed in the work on other prodrugs (Trouet, et al., U.S. Pat. No. 5,962,216), several attempts were made to develop prodrugs of anticancer agents that could be activated by plasmin. The anthracycline daunorubicin (“DNR”) was used as a model drug and several peptidic sequences were linked via their carboxyl end to the amino group of the sugar moiety of the drug.

Based on previous experimental data, it was known that sequences of four amino acid residues would allow the prodrug to be sufficiently stable in the bloodstream and in the biological fluids. The peptide sequences used were selected according to the known substrate specificity of plasmin as described in the literature. In particular, plasmin is a serine protease with trypsin-like specificity, cleaving lysine and arginine bonds. In addition, it is known that plasmin specifically recognizes some peptidic sequences (Lottenberg, et al., Meth. Enzymol. 80:341-361, 1981), and among them D-Alanyl-Leucyl-Lysyl-M and D-Val-Leu-Lys-M, cleaving the peptide bond between the Lysyl residue and the M marker (Smith, et al., Thromb. Res. 17:393, 1980).

As far as plasmin specificity is concerned, several different sequences are possible and have been previously described. Some of these other sequences have been tried for the generation of tripeptide derivatives of anticancer agents to be used as prodrugs, but with very little activity in vivo (Chakravarty, et al. (A), supra; Chakravarty, et al. (B), supra; and Balajthy, et al., supra. These prodrugs were oligopeptidic derivatives of the respective anticancer agent, and were designed in such a way that plasmin action on these derivatives should have regenerated the active compound at the tumor level. However, in the case of anthracyclines (DOX, DNR, etc), these tripeptidic derivatives proved to be very poor substrates for plasmin (D-Val-Leu-Lys-DOX described in Chakravarty, et al. (A), supra and Chakravarty, et al. (B), supra;). More recently, a self-immolative spacer was placed between the tripeptide D-Ala-Phe-Lys to provide prodrugs with relative selectivity to plasmin (de Groot, et al., supra).

In the instant invention, one or two aliphatic amino acids with large lateral side chains, preferably are intercalated between the plasmin substrate sequences described in the art and an agent of interest. In the instant invention, plasmin substrate-Leu-DNR was evaluated, and the effect of modifications of the first N-terminal residue on blood stability was checked. A potentially interesting plasmin-activated prodrug was then selected on the basis of its stability in whole blood and of its degradation into Leu-DNR by purified plasmin as well as by media conditioned by a human breast cancer cell line (MCF-7/6). The structure of this prodrug was D-Ala-Leu-Lys-Leu-DNR (Compound I, DNR). An additional Leu residue was added to provide the prodrug D-Ala-Leu-Lys-Leu-Leu-DNR (Compound II, DNR).

It is understood that other amino acid sequences specifically recognized by plasmin can also be coupled to therapeutic agents in a way to assure an efficient dipeptidyl-agent liberation.

A distinctive feature of the instant invention is the introduction of this single or double aliphatic residue between the specific peptide sequence cleaved off by plasmin and the therapeutic agent, creating a spacer linkage between the enzyme substrate and the drug. It is believed that this spacer linkage facilitates the access of plasmin to the bond that is cleaved. The aliphatic amino acid has a large lateral side chain is preferably a leucine residue. However, other aliphatic amino-acids having a large lateral chain are also suited for use in the invention. These include by way of example and not limitation, phenylalanine, isoleucine and valine residues.

While demonstrated for the drug DNR, the plasmin-sensitive peptide sequences of the invention (D-Ala-Leu-Lys-Leu- and D-Ala-Leu-Lys-Leu-Leu, for example) can be used not only to prepare DNR prodrugs, but also prodrugs of a series of other anticancer agents, as well as of several chemo- or radiosensitizers. In addition, another aspect of the invention involves using this sequence, not only with drugs that are currently used clinically, but also with agents that cannot be used because of their prohibitive toxicity. Therefore, another aspect of the invention relates to a method of significantly reducing the toxicity of an agent while conserving or enhancing its activity.

Stabilizing Group

An important portion of the prodrug is the stabilizing group, which serves to protect the prodrug compound from cleavage in circulating blood when it is administered to the patient and allows the prodrug to reach the vicinity of the target cell relatively intact. The stabilizing group protects the prodrug from cleavage by proteinases and peptidases present in blood, blood serum, and normal tissue. Particularly, in the preferred embodiment, where the stabilizing group caps the N-terminus of the oligopeptide, and is therefore sometimes referred to as an N-cap or N-block, the stabilizing group serves to ward against peptidases to which the prodrug may otherwise be susceptible.

Ideally, the stabilizing group is useful in the prodrug of the invention if it serves to protect the prodrug from degradation, i.e., cleavage, when tested by storage of the prodrug compound in human blood at 37° C. for 2 hours and results in less than 20%, preferably less than 2%, cleavage of the prodrug by the enzymes present in the human blood under the given assay conditions.

More particularly, the stabilizing group can be one of the following:

(1) a non-amino acid group, i.e., a group other than an amino acid; or

(2) an amino acid that is preferably either (i) a non-genetically-encoded amino acid having four or more carbons or (ii) aspartic acid or glutamic acid attached to the N-terminus of the oligopeptide at the β-carboxyl group of aspartic acid or the γ-carboxyl group of glutamic acid; or

(3) a non-amino acid group covalently linked to an amino acid that is preferably either (i) a non-genetically-encoded amino acid having four or more carbons or (ii) aspartic acid or glutamic acid attached to the N-terminus of the oligopeptide at the β-carboxyl group of aspartic acid or the γ-carboxyl group of glutamic acid.

Examples of non-amino acid groups include, by way of example and not limitation, dicarboxylic (or a higher order carboxylic) acid or a pharmaceutically acceptable salt thereof. Since chemical radicals having more than two carboxylic acids are also acceptable as part of the prodrug, the end group having dicarboxylic (or higher order carboxylic) acids is an exemplary N-cap. The N-cap may thus be a monoamide derivative of a chemical radical containing two or more carboxylic acids where the amide is attached onto the amino terminus of the peptide and the remaining carboxylic acids are free and uncoupled. For this purpose, the N-cap is preferably succinic acid, methyl hemisuccinate, adipic acid, glutaric acid, or phthalic acid, with adipic acid and succinic acid being most preferred. Other examples of useful N-caps in the prodrug compound of the invention include diglycolic acid, fumaric acid, naphthalene dicarboxylic acid, pyroglutamic acid, acetic acid, 1 or 2, naphthylcarboxylic acid, 1,8-naphthyl dicarboxylic acid, aconitic acid, carboxycinnamic acid, triazole dicarboxylic acid, gluconic acid, 4-carboxyphenyl boronic acid, PEG and (PEG) _n-analogs, butane disulfonic acid, and maleic acid. Other non-amino acid stabilizing groups include BSA, Boc, Bz and Cbz, Tos, wheat germ agglutinin and succinylated wheat germ agglutinin and dextran.

Suitable amino acid stabilizing groups include, by way of illustration and not limitation, βAla (linked via its carboxyl function to the oligopeptide), α-methyl-Ala, D-Val, D-Ala, D-Phe, D-Ile, D-Pro and poly[N ⁵-(2-hydroxyethyl)-L-glutamine], with β-Ala, α-methyl-Ala and D-Ala being preferred.

Suitable stabilizing groups that are made up of a non-amino acid group covalently linked to an amino acid, include by way of illustration and not limitation, Succ-βAla, Succ-α-methyl-Ala and Succ-D-Ala.

Many cytotoxic compounds inherently have low solubility. Positively charged anthracyclines for example may form aggregates at high concentration and these aggregates may induce intravenous coagulation when the aggregates are administered intravenously. Since many oligopeptides have exposed, positively-charged amino termini at physiological pH, these aggregates may form a polypositively charged surface in vivo and induce a coagulation cascade within a few minutes of administration. This has the potential for rendering any positively charged prodrugs that form aggregates unsuitable for therapeutic use.

One way of addressing such a potentially dangerous obstacle is to utilize the stabilizing group on the peptide chain N-terminus of a negatively charged or a neutral functionality. For example, the use of succinyl as a stabilizing group on the prodrug alleviates the prodrug's acute toxicity. This solves an important problem in the use of peptide prodrugs as practical therapies for intravenous use in humans.

Oligopeptide

Oligopeptides are generally defined as polypeptides of short length. An oligopeptide useful in the prodrug of the invention is 3-6 amino acids in length, however.

As indicated above, the oligopeptide has the formula X-Y, where X is a plasmin peptide substrate of 2-4 amino acids and Y is a peptide comprising 1-2 aliphatic amino acids having large lateral chains. This can also be stated as the formula or sequence (shown in the typical amino-terminus to carboxy-terminus orientation): (AA ^x) _m-(AA ^y) _n(SEQ ID NO:1) wherein: (AA ^x) _mis a plasmin substrate and each AA ^xindependently represents an amino acid; each AA ^yindependently represents an aliphatic amino acid having a large lateral chain; m is an integer from 2-4; and n is an integer from 1-2.

The amino acid sequence of protease cleavage sites is conventionally denoted as H ₂N- . . . -P3-P2-P1-P1′-P2′-P3′- . . . -COOH, where the bond cleaved is between amino acids P1 and P1′ (Schlecter, et al., Biochem. Biophlys. Res. comm. 27:157-162, 1967). Plasmin is a protease with specificity for arginine or lysine at the P1 position, with lysine being preferred. In several known plasmin substrates, P2 is a hydrophobic amino acid while P3 shows no apparent specificity. Accordingly, sequences containing “-amino acid P3-hydrophobic amino acid P2-lysine or arginine P1-” are expected to be good plasmin substrates (Carl, et al., Proc. Natl. Acad. Sci. USA 77(4):2224-2228 (1980).

Therefore, X-Y in its largest embodiment, corresponds to a position sequence —P4-P3-P2-P1-P1′-P2′-, where plasmin cleaves between the P1 and P1′ positions. The “X” plasmin peptide substrate of 2-4 amino acids corresponds to the -P4-P3-P2-P1-section, while the “Y” 1-2 aliphatic amino acid peptide corresponds to the -P1′-P2′-section. Despite the short length of the oligopeptide portion of the prodrug herein described, the selectivity for cleavage of the prodrug by plasmin is maintained.

Preferred Amino Acids

Unless otherwise indicated, all amino acids described herein are in the L configuration.

The following are examples of plasmin substrates suitable for use as the “X” plasmin peptide substrate of the oligopeptide. It is understood that this table is intended to be illustrative and not limiting in any manner.

TABLE 1

				SEQ ID NO:
AA ^x4	AA ^x3	AA ^x2	AA ^x1	(if
(P4)	(P3)	(P2)	(P1)	required)

		Leu	Lys
	Val	Leu	Lys
		Phe	Lys
	Ala	Phe	Lys
		Ala	Lys
	Ala	Ala	Lys
	Leu	Lys	Lys
	Glu	Lys	Lys
Phe	Glu	Lys	Lys	SEQ ID NO:2
		Glu	Lys
	Phe	Glu	Lys
	Ile	Glu	Lys
	Gly	Pro	Lys
		Gly	Arg
	Gly	Gly	Arg
	Val	Gly	Arg
Ile	Glu	Gly	Arg	SEQ ID NO:3
		Pro	Arg
	Gly	Pro	Arg
	Phe	Val	Arg
		Leu	Arg
		Phe	Arg
	Pro	Phe	Arg

Although many amino acids may be present in the oligopeptide portion of the prodrug, certain amino acids are preferred:

Suitable amino acid residues for the P4 or AA ^x4position, include Ile and Phe residues.

Suitable amino acid residues for the P3 or AA ^x3position, include Ala, Glu, Gly, Ile, Leu, Phe, Pro and Val residues.

Suitable amino acid residues for the P2 or AA ^x2position, include Ala, Glu, Gly, Leu, Lys, Phe, Pro and Val residues.

Suitable amino acid residues for the P1 or AA ^x1position, include arginine or lysine residues.

The following are examples of mono- or dipeptides useful for use as the “Y” portion of the oligopeptide. It is understood that this table is intended to be illustrative and not limiting in any manner. Unless otherwise indicated, all amino acids are in the L configuration.

	TABLE 2

	AA ^y1(P1′)	AA ^y2(P2′)

	Leu	—
	Leu	Leu

	*If absent, then Y comprises one amino acid, AA ^y1.

Suitable aliphatic amino acid residues having a large lateral chain for the P1′ or AA ^y1and the P2′ or AA ^y2position, include Ile, Leu, Phe and Val residues. Leu is the preferred residue.

Some preferred oligopeptides useful in the prodrug of the invention include the following, shown in Table 3:

TABLE 3

AA ^x4	AA ^x3	AA ^x2	AA ^x1	AA ^y1	AA ^y2*	SEQ ID NO:
(P4)	(P3)	(P2)	(P1)	(P1′)	(P2′)	(if required)

—	—	Leu	Lys	Leu	—
—	—	Leu	Lys	Leu	Leu	SEQ ID NO:4

*If absent, then Y comprises one amino acid, AA ^y1.

Linker Groups

A linker group between the oligopeptide and the therapeutic agent, although optional, may be advantageous for numerous reasons, such as the following:

- (1) As a spacer for steric considerations in order to facilitate enzymatic release of the AA ^y1or AA ^y2amino acid;
- (2) To provide an appropriate attachment chemistry between the therapeutic agent and the oligopeptide;
- (3) To improve the synthetic process of making the prodrug conjugate (e.g., by pre-derivatizing the therapeutic agent or oligopeptide with the linker group before conjugation to enhance yield or specificity);
- (4) To improve physical properties of the prodrug; and/or
- (5) To provide an additional mechanism for intracellular release of the drug.

Linker structures are dictated by the required functionality. Examples of potential linker chemistries are hydrazide, ester, ether, and sulphydryl. Amino caproic acid is an example of a bifunctional linker group. When amino caproic acid is used in the linker group, it is not counted as an amino acid in the numbering scheme of the oligopeptide. Accordingly, examples of suitable linker groups include amino caproic acid, a hydrazide group, an ester group, an ether group and a sulphydryl group.

Therapeutic Agents

Therapeutic agents that are particularly advantageous to modify to a prodrug form according to the invention are those with a narrow therapeutic window. A therapeutic agent with a narrow therapeutic window is one in which the dose at which toxicity is evident, by general medical standards, is very close to the dose at which efficacy is evident.

The therapeutic agent conjugated to the stabilizing group and oligopeptide and, optionally, the linker group to form the prodrug of the invention may be useful for treatment of cancer, inflammatory disease, or some other medical condition. A series of agents can be considered for use in the invention. In fact, they are the same as those that can be used in the prodrug described in Trouet, et al., U.S. Pat. No. 5,962,216 (βAla-Leu-Ala-Leu-DOX). The main structural requirements concerning these agents is the presence of a reactive amino group or the possibility to introduce such a group without critically decreasing the activity.

In addition to therapeutics agents, the invention can also be used with agents that act as chemosensitizers and dyes. The following exemplify such agents and are intended to be merely illustrative and not limiting in any manner. Preferably, the agent is selected from the following classes of compounds: alkylating agents, anthracyclines, antiproliferative agents, camptothecins, chemotherapeutic agents, cyclosporins, dolastatins, enediynes, epipodophyllotoxins, maytansinoids, naphtalimides, platinum coordination complex, pteridines, rhodamines, sulfoximines, taxanes, taxoids, topoiosomerase inhibitors, tubulin binding agents and vinca alkaloids.

Particularly, the agent is advantageously selected from the following compounds, or a derivative or analog thereof: actinomycin D; alkylating agents such as melphalan; amiodarone; anthracyclines such as daunorubicin and doxorubicin; arabinosides such as cytosine arabinoside and adenosine arabinoside, including 1-β-D-arabinofuranosylcytosine; AT-125 or activin ((αS, 5)-α-amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid); bamipine; bleomycin; 5-bromodeoxyuridine; calicheamicin; camptothecins such as 7-amino-methylcamptothecin; L-canavanine; carboplatin; CC-1065; chlorphenoxamine; chloroquine; colchicine; combretastatin and combretastatin A ₄phosphate; coumarins such as 7-amino-4-trifluoromethylcoumarin and 7-amino-4-methylcoumarin; cyclophosphamide; cyclosporins such as cyclosporin A; cytarabine; dehydrodidemnin B; dipyridamole; discodermolide; docetaxel; dolastatin 10, dolastatin 11 and dolastatin 15; duocarmycin; epothilone A; etoposide and etoposide phosphate; fludarabine; 5-fluorouracil; folic acid derivatives such as aminopterin, methotrexate and dichloromethotrexate; KW-2189; 6-maytansinoids such as maytansine; mercaptopurine; methopterin; mitomycin C; naphtalimides such as amonafide; nicardipine; nitrosoureas such as N-(2-chloroethyl)-N-nitrosourea; paclitaxel; phenylenediamine mustards such as N,N-bis(2-chloroethyl)-p-phenylenediamine; cis-platin; podophyllotoxin and podophyllotoxin derivatives; porfiromycin; quinidine; quinine; reserpine; rhodamines such as rhodamine 123; sulfoximines such as buthionine sulfoximine, methionine sulfoximine and prothionine sulfoximine; tamoxifen; taxoids such as taxotere; topotecan; trifluoperazine; verapamil; vinca alkaloids such as vinblastine and vincristine; as well as derivatives and analogs thereof.

Some preferred prodrugs contemplated by the invention include the following, shown in Table 4. Those having a “D-Ala” stabilizing group are particularly suitable for intraperitoneal administration, while those having a “Succ-D-Ala” stabilizing group are particularly suitable for intravenous administration,:

TABLE 4

Stabilizing	AA ^x4	AA ^x3	AA ^x2	AA ^x1	AA ^y2	AA ^y1*		SEQ ID NO:
Group	(P4)	(P3)	(P2)	(P1)	(P1′)	(P2′)	Drug	(if required)

D-Ala	—	—	Leu	Lys	Leu	—	DNR	SEQ ID NO:5
D-Ala	—	—	Leu	Lys	Leu	—	DOX	SEQ ID NO:5
D-Ala	—	—	Leu	Lys	Leu	Leu	DNR	SEQ ID NO:6
D-Ala	—	—	Leu	Lys	Leu	Leu	DOX	SEQ ID NO:6
Succ-D-Ala	—	—	Leu	Lys	Leu	—	DNR	SEQ ID NO:7
Succ-D-Ala	—	—	Leu	Lys	Leu	—	DOX	SEQ ID NO:7
Succ-D-Ala	—	—	Leu	Lys	Leu	Leu	DNR	SEQ ID NO:8
Succ-D-Ala	—	—	Leu	Lys	Leu	Leu	DOX	SEQ ID NO:8

*If absent, then Y comprises one amino acid, AA ^y2.

Multidrug Resistance

Besides toxicity, drug resistance, a state of decreased sensitivity of cancer cells to therapeutic agents that would ordinarily induce cell death, is another major cause of failure in cancer chemotherapy. Resistance can be intrinsic (no response to initial chemotherapy) or acquired (selection of resistant cells in a population of cancer cells in the course of treatment). Such acquired drug resistance is considered the most common reason for the failure of drug treatment in cancer patients with initially sensitive tumors.

The most critical issue is when multidrug resistance (“MDR”) occurs. In this case, a given cancer cell appears resistant to a series of different anticancer agents, sometimes with quite different structures and/or mechanisms of action. In this case, overcoming the resistance through combination chemotherapy is far more complicated, and research has focused on discovering agents that are likely to inhibit MDR.

Different mechanisms may be responsible for MDR. The most studied and perhaps the most important one involves the amplification and subsequent overexpression of a gene (mdr1) encoding a transmembrane ATP-dependent protein that pumps the drugs out of the cell lowering the effective concentration at the target site. This pump is a 170,000 Da glycoprotein called gp170 or P-glycoprotein. Particularly high levels of this protein are expressed in normal kidney, liver, pancreas, small intestine, colon and adrenal gland where it might play a role in elimination or secretion mechanisms. As the P-glycoprotein transports therapeutic agents of different chemical structure (vinca alkaloids, anthracyclines, epipodophyllotoxins, actinomycin D, etc), it has been proposed to use non-toxic compounds together with the drugs, that would act as competitors for capture by the pump. Several therapeutic agents have been tried such as verapamil, quinine or trifluoperazine, but toxicity and safety continue to remain an issue.

Accordingly, one aspect of the invention pertains to the use of the oligopeptide described herein, having a stabilizing group at its N-terminus (or C-terminus) and verapamil, quinine, trifluoperazine or another suitable P-glycoprotein inhibitor at its C-terminus (or N-terminus) (optionally separated by a linker group), to act as a P-glycoprotein chemosensitizer, i.e., a competitor for capture by the P-glycoprotein pump.

Another important mechanism of MDR affecting anticancer agents such as nitrogen mustard, melphalan, mitomycin, platinum derivatives, etc. involves increased intracellular reducing capacity as reflected by glutathione (“GSH”) levels. GSH normally protects cells by reacting with electrophile derivatives and reactive oxygen species, and in cancer cells, acquired resistance to alkylating agents has been associated with increased GSH. These observations led to the development of inhibitors of γ-glutamylcysteine synthetase (the enzyme involved in GSH biosynthesis) as potential modulators of this type of resistance. Several interesting results have been obtained with one such compound, buthionine sulfoximine. However, due to the important physiological role of GSH, this type of compound is not devoid of toxicity.

Whether considering the implications of P-glycoprotein-mediated or GSH-mediated MDR, it may be preferable to increase the specificity of action of the existing therapeutic agents, rather than to look for other potential modulators. The oligopeptides described herein can be used to obtain prodrugs of multidrug resistance modifiers.

It is particularly important to notice that such modulators of MDR are generally used not as single agents but as part of a chemotherapeutic regimen. Two types of use of the prodrugs of these MDR modulators are contemplated by the invention: (i) use of these prodrugs together with classical treatments, and (ii) use of these prodrugs with treatments based on the less toxic prodrugs of the classically used anticancer agents described herein. The latter type of use could even lead to better results on resistant cells, since the prodrugs of the anticancer agents are supposed to lead to higher intracellular drug concentrations.

Accordingly, another aspect of the invention pertains to the use of the oligopeptide described herein, having a stabilizing group at its N-terminus (or C-terminus) and buthionine sulfoximine or another suitable γ-glutamylcysteine synthetase inhibitor at its C-terminus (or N-terminus) (optionally separated by a linker group), to inhibit GSH production.

Both the (stabilizing group)-(oligopeptide)-(optional linker group)-(P-glycoprotein inhibitor) and the (stabilizing group)-(oligopeptide)-(optional linker group)-(γ-glutamylcysteine synthetase inhibitor) described above would find utility in methods of treating a patient.

Screening of the Prodrug

The synthesized prodrug can be tested against a test standard such as the plasmin substrate, D-Ala-Leu-Lys, conjugated to a marker, 7-amido-4-methylcoumarin. The rates of hydrolysis of the synthesized peptidyl prodrug and the test standard by plasmin are compared under common experimental conditions. The General Methods, Section N., below, provides an exemplary scheme for performing this test.

The disclosure of making and using the prodrugs taught herein provides a useful alternative to prior teachings of prodrug design. As illustrated in the examples below, the prodrugs of the invention are efficacious and well-tolerated iii vivo in animal models. As such, the prodrugs are advantageously utilized in therapy.

Prodrug Design

A method of designing a prodrug is another aspect of the invention and entails initially selecting a peptide of two-five amino acids, then adding one or two amino acids having large aliphatic side chains (e.g., Leu) to form an oligopeptide. The oligopeptide is linked at a first attachment site of the oligopeptide to a stabilizing group that hinders cleavage of the oligopeptide by enzymes present in whole blood, and directly or indirectly linked to a therapeutic agent at a second attachment site of the oligopeptide. The linkage of the oligopeptide to the therapeutic agent and the stabilizing group may be performed in any order or concurrently. The resulting conjugate is tested for cleavability by plasmin under given experimental conditions. The standard rate of cleavage is tested on a test standard by plasmin under the same given experimental conditions. The test standard consists of a conjugate of D-Ala-Leu-Lys and the marker, 7-amido-4-methylcoumarin. The first attachment site is usually the N-terminus of the oligopeptide but may be the C-terminus of the oligopeptide or another part of the oligopeptide. The second attachment site is usually the C-terminus of the oligopeptide, but may be the N-terminus of the oligopeptide or another part of the oligopeptide. A prodrug designed by such a method is also part of the invention.

Further, the invention includes a method for decreasing toxicity of a therapeutic agent that is intended for administration to a patient. Specifically, a modified, prodrug form of the therapeutic agent is formed by directly or indirectly linking the therapeutic agent to an oligopeptide that is cleavable by plasmin under physiological conditions. The prodrug provides for decreased toxicity of the therapeutic agent when administered to the patient. The modification of the therapeutic agent in this manner also allows for administration of an increased dosage of the therapeutic agent to the patient relative to the dosage of the therapeutic agent in unconjugated form.

Pharmaceutical Compositions

The invention also includes a pharmaceutical composition comprising a compound, particularly a prodrug compound, according to the invention and, optionally, a pharmaceutically acceptable adjuvant or vehicle.

The invention also relates to the use of the pharmaceutical composition for the preparation of a medicinal product intended for the treatment of a medical condition.

The pharmaceutical composition may, for example, be administered to the patient parenterally, especially intravenously, intramuscularly, or intraperitoneally. Pharmaceutical compositions of the invention for parenteral administration comprise sterile, aqueous or nonaqueous solutions, suspensions, or emulsions. As a pharmaceutically acceptable solvent or vehicle, propylene glycol, polyethylene glycol, injectable organic esters, for example ethyl oleate, or cyclodextrins may be employed. Isotonic saline may be part of the pharmaceutical composition. These compositions can also comprise wetting, emulsifying and/or dispersing agents.

The sterilization may be carried out in several ways, for example using a bacteriological filter, by incorporating sterilizing agents in the composition or by irradiation. They may also be prepared in the form of sterile solid compositions, which may be dissolved at the time of use in sterile water or any other sterile injectable medium.

The pharmaceutical composition may also comprise adjuvants that are well known in the art (e.g., vitamin C, antioxidant agents, etc.) and capable of being used in combination with the compound of the invention in order to improve and prolong the treatment of the medical condition for which they are administered.

Doses for administration to a patient of the compounds according to the invention are generally at least the usual doses of the therapeutic agents known in the field, described in Chabner, et al., Cancer Chemotherapy (Lippincott ed., ISBN 0-397-50900-6, 1990) or they may be adjusted, within the judgment of the treating physician, to accommodate the superior effectiveness of the prodrug formulations or the particular circumstances of the patient being treated. Hence, the doses administered vary in accordance with the therapeutic agent used for the preparation of the compound according to the invention.

Treatment of Patients with Prodrug Compound

A method for the therapeutic treatment of a medical condition that involves administering, preferably parenterally and more preferably intravenously, to the patient a therapeutically effective dose of the pharmaceutical composition is also within the scope of the invention. Thus, the method generally entails administering to the patient a therapeutically effective amount of a compound comprising:

(1) a therapeutic agent capable of entering a target cell;

(2) an oligopeptide having the formula X-Y, where X is a plasmin peptide substrate of 2-4 amino acids and Y is a peptide comprising 1-2 aliphatic amino acids having large lateral chains;

(3) a stabilizing group; and

(4) optionally, a linker group not cleavable by plasmin;

wherein the stabilizing group hinders cleavage of the oligopeptide by enzymes present in whole blood; and

wherein the compound is cleaved by plasmin.

The oligopeptide can also be described as having the formula or sequence: (AA ^x) _m-(AA ^y) _n(SEQ ID NO:1) wherein: (AA ^x) _mis a plasmin substrate and each AA ^xindependently represents an amino acid; each AA ^yindependently represents an aliphatic amino acid having a large lateral chain; m is an integer from 2-4; and n is an integer from 1-2.

The prodrug compound is useful for the treatment of many medical conditions including cancer, neoplastic diseases, tumors, inflammatory diseases, and infectious diseases. Examples of preferred diseases are breast cancer, colorectal cancer, liver cancer, lung cancer, prostate cancer, ovarian cancer, brain cancer, and pancreatic cancer. Formulated in pharmaceutically acceptable vehicles (such as isotonic saline), the prodrug compound can be administered to animals or humans in intravenous doses ranging from 0.05 mg/kg/dose/day to 300 mg/kg/dose/day. It can also be administered via intravenous drip or other slow infusion method.

Human patients are the usual recipients of the prodrug of the invention, although veterinary usage is also contemplated.

Diagnosis or Assay

An article of manufacture, such as a kit, for diagnosis or conducting an assay is also within the scope of the invention. Such an article of manufacture would preferably utilize a compound as described above, except that a marker, such as coumarin, is conjugated to the oligopeptide and stabilizing group instead of a therapeutic agent. At least one reagent useful in the detection of the marker is typically included as part of the kit. Thus, the article of manufacture would include the following:

(1) a compound comprising:

(a) a marker;

(b) an oligopeptide having the formula X-Y, where X is a plasmin peptide substrate of 2-4 amino acids and Y is a peptide comprising 1-2 aliphatic amino acids having large lateral chains;

(d) optionally, a linker group not cleavable by plasmin;

wherein the stabilizing group hinders cleavage of the oligopeptide by enzymes present in whole blood; and

wherein the compound is cleavable by plasmin; and

(2) at least one reagent useful in the detection of the marker.

As noted above, the standard rate of cleavage is tested on a test standard by plasmin under the same experimental conditions and the test standard consists of a conjugate of a plasmin substrate and a marker, for example D-Ala-Leu-Lys-7-amido-4-methylcoumarin. Further, the oligopeptide can also be described as having the formula or sequence (AA ^x) _m-(AA ^y) _n.

The article of manufacture may be used, for example, with patient samples to diagnose tumors or to identify patients susceptible to treatment by prodrug therapy.

Process Chemistry General Procedures

Oligopeptide: General Method for the Synthesis of Peptides

The peptide or oligopeptide sequences in the prodrug conjugates of this invention may be synthesized by the solid phase peptide synthesis (using either Boc or Fmoc chemistry) methods or by solution phase synthesis. The general Boc and Fmoc methods are widely used and are described in the following references: Merrifield, J. A. Chem. Soc., 88:2149, 1963; Bodanszky and Bodanszky, The Practice of Peptide Synthesis , Springer-Verlag, Berlin, 7-161, 1994; Stewart, Solid Phase Peptide Synthesis , Pierce Chemical, Rockford, 1984.

General Fmoc Solid Phase Method

Using the preferred solid phase synthesis method, either automated or manual, a peptide of desired length and sequence is synthesized through the stepwise addition of amino acids to a growing chain which is linked to a solid resin. Examples of useful Fmoc compatible resins include, but are not limited to, Wang Resins (Wang, J. Am. Chem. Soc. 95:1328, 1973 and Zhang, et al., Tet. Lett. 37:5457, 1996), HMPA-PEGA resin, Rink Resins (Rink, Tet. Lett. 28:3787, 1987), or a hydroxyethyl-photolinker resin. The C-terminus of the peptide chain is covalently linked to a polymeric resin and protected α-amino acids were added in a stepwise manner with a coupling reagent. A preferred α-amino protecting group is the Fmoc group, which is stable to coupling conditions and can readily be removed under mild alkaline conditions. The reaction solvents are preferably but not limited to DMF, NMP, DCM, MeOH and EtOH. Examples of coupling agents are: DCC, DIC, HATU and HBTU. Cleavage of the N-terminal protecting group is accomplished in 10-100% piperidine in DMF at 0-40° C., with ambient temperature being preferred. At the end of synthesis, the final Fmoc protecting group is removed using the above N-terminal cleavage procedure. The remaining peptide on resin is cleaved from the resin along with any acid sensitive side chain protecting groups by treating the resin under acidic conditions. For example, an acidic cleavage condition is a mixture of TFA in DCM. If the hydroxyethyl-photolinker resin is used, the appropriate wavelength for inducing cleavage is λ365 nm ultraviolet light.

General N-cap Method via Solid Phase Synthesis

The preparation of N-terminus derivatized peptides is conveniently accomplished on a solid phase. When the peptide synthesis is complete, the terminal Fmoc is removed while the peptide is still on the solid support. The N-cap of choice is coupled next using standard peptide coupling conditions onto the N-terminus of the peptide. On completion of the N-cap coupling the peptide is cleaved from the resin using the procedure described above.

General Boc Solid Phase Method

For the solid phase method using Boc chemistry, either the Merrifield resin or PAM resin is useful. The amino acids are coupled to the growing chain on solid phase by successive additions of coupling agent activated Boc-protected amino acids. Examples of coupling agents are: DCC, DIC, HATU and HBTU. The reaction solvents may be DMF, DCM, MeOH and NMP. Cleavage of the Boc protecting group is accomplished in 10-100% TFA in DCM at 0-40° C., with ambient temperature being preferred. On completion of the peptide chain assembly the N-terminus protecting group (usually Boc) is removed as described above. The peptide is removed from the resin using liquid HF or trifluoromethane sulfonic acid in DCM. Schemes I and II illustrate the Merrifield solid phase peptide synthesis (“SPPS”) method.

embedded image

N-α-Protecting Boc of the amino acid directly attached to the Boc-Leu-Merrifield Resin (1) is first cleaved in the vessel for manual SPPS by vigorous shaking with a mixture of TFA:CH ₂Cl ₂(1:1, v/v) for 30 min at RT to yield NH ₂-Leu-Merrified Resin (2). This procedure, called Boc-deprotection can be performed as follows (solvent volumes given for 10 g of resin).


CH ₂Cl ₂	60 ml	3 × 1 min
TFA:CH ₂Cl ₂(1:1)	50 ml	1 × 1 min
TFA:CH ₂Cl ₂(1:1)	50 ml	1 × 30 min
CH ₂Cl ₂	50 ml	6 × 1 min
DIPEA 5% (v/v) in CH ₂Cl ₂	50 ml	3 × 2 min
CH ₂Cl ₂	50 ml	6 × 1 min

Step B: N-protected Amino Acid Attachment

embedded image

3 eq. of the Boc-protected amino acid to attach next, for example, Boc-Lys(Fmoc)-OH, is dissolved in CH ₂Cl ₂(45 ml) and put into contact with the resin (2) under shaking for 10 min. 3 eq. of DCC is dissolved in CH ₂Cl ₂(5 ml) and added into the reaction mixture. The latter is agitated for 2 hours at RT to produce Boc-Lys(Fmoc)-Leu-Merited Resin (3). The reaction is then checked by the ninhydrin test for completeness.

The ninhydrin test uses three solutions: Solution A contains 50 mg ninhydrin in 10 ml EtOH; Solution B contains 80 mg phenol in 20 ml EtOH; and Solution C contains 2 ml KSCN 0.01M in H ₂O in 100 ml pyridine. A few milligrams of the product to be tested is introduced in a test tube, and two drops of Solution A, two drops of Solution B, and two drops of Solution C are added. The mixture is heated in a boiling water bath for five min and 1 ml of EtOH is added. If the reaction is complete, the liquid remains colorless. Blue coloration of the material indicates the presence of a free amino groups with an intensity proportional to their concentration.

If the ninhydrin test is negative (i.e., colorless), the Boc-attachment step is considered to be complete. If positive (i.e., blue), a new coupling procedure step with the same quantity of reagents is repeated.

Step C: Attachment of Amino Acids

Attachment of subsequent amino acids involves repeating Steps A and B, as needed:

embedded image
where “MR” is the Merrifield Resin.

The final coupling step with this technique generally includes a Fmoc-protected amino acid, designed to terminate the sequence the be synthesized (the same proportions of reagents and procedures being employed as in the case of Boc-protected amino acids).

Step D: Cleavage of the Fmoc-protected Tetrapeptide (Fmoc-D-Ala-Leu-Lys(Fmoc)-Leu-OH) from the Resin

The Fmoc-protected peptide is removed from the resin by the addition of HBr (30% in MeOH):TFA (1:1) for 30 min. This is followed by treatment with TFA (3×) and CH ₂Cl ₂(6×).

Alternately, the Fmoc-protected peptide removal from the resin can be achieved by incubating the product in the following solutions:


CH ₂Cl ₂	50 ml	1 × 10 min	discard
TFA:CH ₂Cl ₂(1:1, v/v)	50 ml	1 × 10 min	discard
TFA	50 ml	1 × 30 min	discard
TFA	50 ml	1 × 30 min	discard
TFA:HBr (1:1, v/v)	60 ml	1 × 30 min	recover
TFA	50 ml	3 × 3 min	recover
CH ₂Cl ₂	50 ml	→ colorless	recover

Step E: Solvent Evaporation Dissolution in MeOH and Precipitation in H ₂O

The solvent is repeatedly evaporated (the solid redissolved in CH ₂Cl ₂) until the disappearance of an acid odor (at least 5 times). The product is dissolved in MeOH. A large quantity of ice-cold water (at least 95% of the resulting volume, w/v) is then added, the mixture stirred and the precipitate filtered on frit glass. The Fmoc-protected peptide is then lyophilized.

The following scheme provides the synthesis route for Compounds I and II, using DNR HCl or DNR-Leu HCl, respectively, as the starting materials. It is understood, however, that other anthracycline prodrugs and prodrugs of other therapeutic agents can be synthesized in a similar manner, or by techniques that are well known to those of skill in the art.

embedded image
General Procedure for the Preparation of Fmoc Oligopeptide by Solution Phase Synthesis

Alternatively, the prodrug peptide intermediate may be made via a solution phase synthesis, utilizing either Boc or Fmoc chemistry. The peptide can be built up by the stepwise assembly in analogy to the solid phase method (in the N-terminal direction or in the C-terminal direction) or through the coupling of a dipeptide with a single amino acid.

One method of solution phase synthesis is a stepwise building up of the prodrug peptide intermediate using Fmoc chemistry. The C-terminus must first be protected to reduce the formation of side products. The C-terminal group in is typically Me, tBu, Bz or TCE. (Note when the N-cap is methyl succinyl the C-terminus group cannot be methyl.). DMF is a common solvent, as are DMSO, CH ₃CN and NMP (or mixtures thereof). Pyridine, TEA or other bases may be substituted for piperidine in deprotecting the growing peptide chain protected amino terminus. HBTU is a common activating agent, but other activating agents such as DCC, DIC, DCC+HOBT, OSu, activated esters, azide, or triphenyl phosphoryl azide can also be used. Additionally, the protected peptide acid chloride or acid bromide may be used to couple directly to the amino acid or peptide fragment. On completion of the oligopeptide assembly, the N-terminus is deprotected and the C-terminus protected peptide is ready to accept the desired N-cap.

General Procedure for the Preparation of N-cap Oligopeptide via Solution Phase Synthesis

When constructing the N-capped oligopeptide by solution phase synthesis, the N-cap needs to be synthesized by a slightly modified procedure. First the C-terminus of the Fmoc oligopeptide needs to be protected with an acid labile or hydrogenation sensitive protecting group compatible with the selective deprotection of the C-terminus over the N-cap. Then the Fmoc protecting group needs to be removed from the oligopeptide to reveal the N-terminus. With the N-terminus deprotected and the C-terminus protected, the oligopeptide is reacted with the activated hemiester of the desired N-cap. The N-cap can be activated using methods for activating amino acids such as DCC or HATU in base and an appropriate solvent. Alternatively, where MeOSucc is used, the coupling may also be done via methyl hemisuccinyl chloride (or other acid halide) using an inert solvent in the presence of an organic or inorganic base, such as DIPEA, TEA or Cs ₂CO ₃. One example of such a synthesis can be by reacting MeOSucc and (Amino acid) _i-benzyl ester. The coupling method can be any one of the methods generally used in the art (see for example: Bodanszky, The Practice of Peptide Synthesis , Springer Verlag, 185, 1984; and Bodanszky, Principles of Peptide Synthesis , Springer Verlag, 159, 1984. The benzyl group then can be removed by catalytic hydrogenation providing the desired N-cap methylsuccinyl form of the oligopeptide. Other examples of suitable, selectively removable C-terminal protecting groups can be, but are not limited to, tBu, alkoxy-methyl and TCE. Other methods of accomplishing this step are described in the literature.

The reaction conditions are well known in the art and detailed in the citations given. The advantage of the above described methods is the facile purification of the product produced by solution phase synthesis.

Prodrug Conjugate

General Methods for the Conjugation and Deprotection Steps

The prodrugs described herein can be synthesized by coupling an Fmoc form (which means Fmoc is attached to the N-terminus of the oligopeptide) of the oligopeptide with daunorubicin, or doxorubicin, or any appropriate therapeutic agent using any of the standard activating reagents used in peptide synthesis. The solvent may be toluene, ethyl acetate, DMF, DMSO, CH ₃CN, NAP, THF, DCM or any other suitable inert solvent as is known in the art and the reagents are soluble therein. Preferred solvents include DMF and NMP. The appropriate temperature range is −25 to +25° C., with ambient temperature being preferred. The activating agent may be selected from one of the following: PyBOP, HBTU, HATU, DIC, DCC, DCC+HOBT, OSu activated esters, azide, or triphenylphosphorylazide. HBTU or HATU are preferred activating agents. Alternatively, the acid chloride or the acid bromide of the protected peptide can also be used for this coupling reaction. 2-4 equivalents, advantageously 2-2.5 equivalents of a base is required for the coupling reaction. The base can be selected from inorganic bases such as CsCO ₃, Na— or K ₂CO ₃, or organic bases, such as TEA, DIPEA, DBU, DBN, DBO, pyridine, substituted pyridines, N-methyl-morpholine etc., preferably TEA, or DIPEA. The reaction can be carried out at temperatures between −15° C. and 50° C., advantageously between −10° C. and 10° C. The reaction time is between 5-90 minutes and is advantageously 20-40 minutes. The product is isolated by pouring the reaction mixture into water and filtering the precipitate formed. The crude product can be further purified by recrystallization from DCM, THF, ethyl acetate, or ACN, preferably from DCM or ACN. The isolated Fmoc form of the (oligopeptide)-(therapeutic agent) conjugate is then deprotected over 2-90 minutes, preferably 3-8 minutes, using a ten- to hundred-fold excess of base at a temperature between −10° C. and 50° C. Ideally, 5-60 equivalents of the base are preferred. Piperidine is the preferred base to deprotect Fmoc groups. The deprotected amino terminus of the (oligopeptide)-(therapeutic agent) conjugate is acylated by a diacid anhydride as an activated hemiester to give the final N-cap form of the oligopeptide-therapeutic agent.

Alternatively, the final prodrug can be similarly prepared from the protected N-cap form of the oligopeptide such as a methylhemiester form of succinyl-N-cap oligopeptide and conjugated to a therapeutic agent.

The (protected N-Cap)-(oligopeptide)-(therapeutic agent) conjugate is now deprotected by methods compatible to the stability of the therapeutic agent. For other therapeutic agents, benzyl protecting groups and catalytic hydrogenation to deprotect might be chosen.

Conversion to the salt form of the negatively charged (N-cap)-(oligopeptide)-(therapeutic agent) is carried out with a solvent selected from the following group: alcohol (including MeOH, EtOH or isopropanol), water, ACN, THF, diglyme or other polar solvents. The sodium source is one molar equivalent of NaHCO ₃, NaOH, Na ₂CO ₃, NaOAc, NaOCH ₃(in general sodium alkoxide), or NaH. An ion exchange column charged with Na ⁺ (such as strong or weak ion exchangers) is also useful for this last step of making the salt form of the (N-cap)-(oligopeptide)-(therapeutic agent) when appropriate. Sodium is described in this application as an example only.

Generally, the prodrug may be converted to a pharmaceutically acceptable salt form to improve solubility of the prodrug. The (N-cap)-(oligopeptide)-(therapeutic agent) is neutralized with a pharmaceutically acceptable salt, e.g., NaHCO ₃, Na ₂CO ₃, NaOH tris(hydroxymethyl)-aminomethane, KHCO ₃, K ₂CO ₃, CaCO ₃, NH ₄OH, CH ₃NH ₂, (CH ₃) ₂NH, (CH ₃) ₃N, acetyltriethylammonium. The preferred salt form of prodrug is sodium and the preferred neutralizing salt is NaHCO ₃.

It is well documented that anthracycline type molecules, including doxorubicin and daunorubicin form gels in organic solvents in very low concentrations (Matzanke, et al., Eur. J. Biochem. 207:747-55, 1992; Chaires, et al., Biochemistry 21:3927-32, 1982; and Hayakawa, et al., Chem. Pharm. Bull. 39:1282-6, 1991). This may be a considerable obstacle to getting high yields of clean product when making peptide anthracycline conjugates. The gel formation contributes to the formation of undesirable side reactions. One way to minimize this problem is to use very dilute solutions (1-2%) for the coupling reaction, however it is not practical in a process environment (large amounts of waste, complicated isolation). To overcome this problem, urea or other chaotropic agents may be used to break up the strong hydrophobic and hydrogen bonding forces forming the gel. Thus if the coupling reaction is carried out in a urea-containing solvent, advantageously a 20% to saturated solution of urea in DMF or NMP, the side reactions can be kept below 2% even if the concentration of reactants exceeds 10%. This procedure makes the conjugation step practical at high concentrations and produces good yields and improved purity over the procedures that do not use urea or other chaotropic agents.

General Enzyme Method

Hydrolysis of protected N-cap-oligopeptide therapeutic agents to the full N-cap compound catalyzed by acids or bases leads to complex reaction mixtures due to the lability of many therapeutic agents even under moderately acidic or basic conditions. Enzymes can promote the hydrolysis without destroying the substrate or the product. Enzymes suitable for this reaction can be esterases or lipases and can be in their natural, water soluble forms or immobilized by cross coupling, or attachment to commercially available solid support materials. Of the soluble enzymes evaluated, Candida Antarctica “B” lipase (Altus Biologics) is especially useful example of an enzyme immobilized by cross coupling is ChiroCLEC-PC™ (Altus Biologics). Candida Antarctica “B” lipase (Altus Biologics) can be immobilized by reaction with NHS activated Sepharose™ 4 Fast Flow (American Pharmacia Biotech). The pH of the reaction mixture during the hydrolysis is carefully controlled and maintained by a pH-stat between 5.5 and 7.5, advantageously between 5.7 and 6.5, via controlled addition of NaHCO ₃solution. When the reaction is completed the product is isolated by lyophilization of the filtered reaction mixture. The immobilized enzymes remain on the filter cake and can be reused if desired.

General Allyl or Alkyl Ester Method

The prodrug can also be prepared via coupling an allyl-hemiester or alkyl-hemiester form of the N-cap oligopeptide with a therapeutic agent and then liberating the free acid from the conjugate. The coupling of allyl-succinyl-(Amino acid) _i-with doxorubicin can be carried out via any one of the oligopeptide conjugation methods.

Allyl-succinyl-(Amino acid) _i-doxorubicin can also be synthesized by reacting allyl hemisuccinate, which is prepared via known methods (Casimir, et al., Tet. Lett. 36:19,3409, 1995), with (Amino acid) _i-doxorubicin similarly as coupling of the protected peptidyl precursors to doxorubicin was described in the previous methods. Suitable inert solvents are THF, DCM, ethyl acetate, toluene, preferably THF from which the acid form of the product precipitates as the reaction progresses. The isolated acid is converted to its sodium salt as described earlier. Reaction times vary between 10-180 minutes, advantageously 10-60 minutes, at temperatures between 0-60° C., preferably 15-30° C.

Removal of the allyl or alkyl group can be done with Pd(0), or Ni(0), advantageously Pd(0) promoted transfer of the allyl or alkyl group to acceptor molecules, as it is well known in the art and documented in the professional literature (Genet, et al., Tet. Lett. 50:497, 1994; Bricout, et. al., Tet. Lett. 54:1073, 1998, Genet, et. al., Synlett 680, 1993; Waldmann, et. al., Bioorg. Med. Chem. 7:749, 1998; Shaphiro, et al., Tet. Lett. 35:5421, 1994). The amount of catalyst can be 0.5-25 mol % to the substrate.

General Trityl or Substituted Tritel Method

The prodrug may also be synthesized by utilizing an R′-oligopeptide, where R′ is trityl or substituted trityl. The coupling of R′-oligopeptide with a therapeutic agent can be carried out via any one of the methods described earlier for conjugation of a protected oligopeptide with a therapeutic agent at 30-120 minutes at 0-20° C.

Removal of trityl or substituted trityl group can be achieved under acidic conditions to give the positively charged prodrug, which is N-capped as described above. The trityl deprotection can be accomplished with acetic acid, formic acid and dilute hydrochloric acid.

The prodrug can be converted into (succinyl or glutaryl)-(oligopeptide)-(therapeutic agent) conjugate by reacting with succinic anhydride or glutaric anhydride. The solvent for coupling step DMF, DMSO, CH ₃CN, NMP, or any other suitable solvent is known in the art. Succinyl or glutaryl oligopeptide therapeutic agents can be converted to any pharmaceutically acceptable salt.

General Inverse Direction Solid Phase Conjugation Method

The prodrug compound of the present invention can be synthesized by using solid phase chemistry via “step wise” inverse (from the N-terminal to the C-terminal) direction methods.

One way is to use resins to immobilize a succinyl hemiester, for example succinyl-mono-benzyl ester or -allyl ester. Examples of resins could be selected are Wang Resins; Rink Resins; and Trityl-, or substituted-trityl Resins (Chen, et. al., J. Am. Chem. Soc. 116:2661, 1994; Bartos, et. al., Peptides, Proc. 22 ^ndEuropean Peptide Symposium, 1992; and Schneider, et al., (Eds.), ESCOM, Leiden, pp. 281, 1993). The immobilized ester is then deprotected and reacted with, for example, a similarly C-terminal protected methionine. These steps are then repeated with appropriate amino acid residues, followed by the coupling of doxorubicin to the immobilized succinyl-tripeptide. The molecule is then liberated from the resin by using mildly acidic conditions to form a free prodrug, such as free Succ-(Amino acid) _i-Leu-DOX. Another version of phase synthesis utilizes immobilized succinyl oligopeptide ester. This is then C-terminally deprotected, followed by the coupling step to doxorubicin or other therapeutic agent, and finally liberated from the resin. The acid form of the prodrug molecule may then be converted finally into its sodium salt as described above.

Removal of Free Therapeutic Agent

Unconjugated therapeutic agent may be present late in the process of making the prodrug. For example, during the coupling step of (stabilizing group)-(oligopeptide) conjugate with doxorubicin as the therapeutic agent, it has been found in some instances, that the reaction does not proceed completely. Initial attempts to remove doxorubicin completely can be attempted by acidic washes. In the event that such attempts do not result in complete removal, any remaining free therapeutic agent can be removed by a process that utilizes scavenging resin or beads.

The crude product, which contains the intermediate and residual doxorubicin, is dissolved in DMF and polystyrene methylisocyanate or polystyrene sulfonyl chloride resin or beads are added. The reaction is then stirred for 60 minutes. The free amino group of doxorubicin reacts with the isocyanate or sulfonyl chloride group on the beads to form a urea or sulfonamide derivative. The solid beads with doxorubicin attached to them are then separated from the desired product by filtration. The desired product remains in the DMF solution. This approach seems to be a very mild and effective method for removing residual therapeutic agent from the product.

Thus, the invention includes a method of making a compound comprising:

- (1) selecting an Fmoc-protected oligopeptide having a formula, Fmoc-(AA ^x) _m-(AA ^y) _n, where AA ^x, AA ^y, m and n are as defined above;
- (2) coupling the Fmoc-protected oligopeptide to a therapeutic agent by activating the Fmoc-protected oligopeptide with an activating agent in the presence of the therapeutic agent to form an Fmoc-protected oligopeptide-therapeutic agent conjugate;
- (3) deprotecting the Fmoc-protected oligopeptide-therapeutic agent conjugate by contacting it with a base to form an oligopeptide-therapeutic agent conjugate; and
- (4) coupling the oligopeptide-therapeutic agent conjugate to a stabilizing group to form the compound.

Alternatively, a method of making a compound comprises the following steps:

- (1) selecting an oligopeptide having the formula, (AA ^x) _m-(AA ^y) _n, where AA ^x, AA ^y, m and n are as defined above;
- (2) coupling the oligopeptide to an alkyl ester-protected stabilizing group to form an alkyl ester-protected stabilizing group-oligopeptide conjugate;
- (3) coupling the alkyl ester-protected-stabilizing group-oligopeptide conjugate to a therapeutic agent by activating the alkyl ester-protected stabilizing group-oligopeptide conjugate with an activating agent in the presence of a therapeutic agent to form an alkyl ester-protected stabilizing group-oligopeptide-therapeutic agent conjugate; and
- (4) deprotecting the alkyl ester-protected stabilizing group-oligopeptide therapeutic agent conjugate to form the compound.

A compound of the invention may also be made via the following steps:

- (1) selecting an oligopeptide having the formula, (AA ^x) _m-(AA ^y) _n, where AA ^x, AA ^y, m and n are as defined above;
- (2) coupling the oligopeptide to an allyl ester-protected stabilizing group to form an allyl ester-protected stabilizing group-oligopeptide conjugate;
- (3) coupling the allyl ester-protected-stabilizing group-oligopeptide conjugate to a therapeutic agent by activating the allyl ester-protected stabilizing group-oligopeptide conjugate with an activating agent in the presence of a therapeutic agent to form an allyl ester-protected stabilizing group-oligopeptide-therapeutic agent conjugate; and
- (4) deprotecting the allyl ester-protected stabilizing group-oligopeptide therapeutic agent conjugate to form the compound.

Yet another method for making a compound of the invention comprises the following steps:

- (1) selecting a trityl-protected oligopeptide having a formula, trityl-(AA ^x) _m-(AA ^y) _n, where AA ^x, AA ^y, m and n are as defined above;
- (2) coupling the trityl-protected oligopeptide to a therapeutic agent by activating the trityl-protected oligopeptide with an activating agent in the presence of a therapeutic agent, thereby making a trityl-protected oligopeptide-therapeutic agent conjugate;
- (3) deprotecting the trityl-protected oligopeptide-therapeutic agent conjugate under acidic conditions to form an oligopeptide-therapeutic agent conjugate; and
- (4) coupling the oligopeptide-therapeutic agent conjugate with an stabilizing group to form the compound.

Another possible step in connection with any of these methods is removing uncoupled therapeutic agent by use of scavenging resin or beads. Further, the compound may be neutralized with a pharmaceutically acceptable salt if desired.

The present invention will be described in greater detail in the examples which follow, given by way of non-limiting illustration of the present invention.

EXAMPLES

General Methods

A. Synthesis of Amino Acid Derivatives of DNR by a Progressive Elongation of the Peptidic Sequence

1. Products

DNR-HCl; Fmoc-protected amino acids (Novabiochem); DIPEA (98+%, Acros); DMF (Uvasol for spectroscopy, Merck); HATU (97%, Aldrich); apyrogen water (Baxter); lactate buffer (L-Lactic acid in 85% water, Aldrich and NaOH 1N, Vel); piperidine (>99%, Fluka); chloroform (analytical reagent, Labscan); sodium sulfate (Vel); MeOH (analytical reagent, Labscan); Et ₂O (>99.8%, Fluka); N-hexane (99%, Labscan); glutaric anhydride (97%, Acros); C18 ODS-A silica gel (40-60 μm, 120 Å, YMC).

2. Synthesis of (Amino Acid) _n+1-DNR

Piperidine (50.0 eq.) was added to a solution of Fmoc-(Amino acid) _n+ ₁-DNR in DMF (50 ml per mmole DNR). After stirring for 5 min at RT, the mixture Et ₂O:N-hexane(1:1, 6 ml per mmole DNR) was added. The precipitate was filtered in a glass fit, thoroughly washed with the same mixture and dissolved in chloroform (6 ml per mmole DNR). The product was purified by silica gel column (mobile phase chloroform=MeOH, <10%), the solvent evaporated and its purity assessed by HPLC (See General Methods, Section D.1.).

The residue was either used, without further purification, for further synthesis with Fmoc-protected amino acids or was dissolved in water by adding HCl dropwise (0.1M water solution), pH controlled (>4.0) and lyophilized.

B. TLC Method for Qualitative Analysis of DNR/DOX Peptidic Derivatives

TLC analysis was carried out on silica gel 60F-254 nm-0.25 mm plates (Merck) with DCM:MeOH:H ₂O:Formic acid 88% (85:15:1:2, v/v) for elution.

C. HPLC Method #1 for Purity Analysis of Fmoc-Containing Peptidic Derivatives of DNR and DOX

The column was TSK Gel Super ODS (ref. 18197, 2 μm). Solvent A was TFA 0.1% in water (w/v), and Solvent B was TFA 0.1% in ACN (v/v).

Method: 30-36% of Solvent B in 2 min, 36-41% of Solvent B in 10 min, 41-90% of Solvent B in 3 min, 5 min at 90% Solvent B. Flow rate of 1.000 ml/min. UV detection at 254 nm. Fluorescence detection with ex. λ of 480 and em. λ of 560 nm. Purity of the compounds was assessed as the percentage surface area of the peaks.

D. HPLC Method #2 for Purity Analysis of Peptidic Derivatives of DNR and DOX

1. DNR

The column was Luna C18 3 μm, 4.6×100 mm ID (ref. 00d-4251-e0). Solvent A was TFA 0.1% in water (w/v), and Solvent B was TFA 0.1% in ACN (v/v).

Method: 31% of Solvent B for 5 min, 31-43% of Solvent B in 3 min, 43-90% of Solvent B in 0.5 min, 2.5 min at 90% Solvent B. Flow rate of 1.500 ml/min. UV detection at 254 nm. Fluorescence detection with ex. λ of 480 and em. λ of 560 nm. Purity of the compounds was assessed as the percentage surface area of the peaks.

2. DOX

The column was Symmetry shield waters RP8 3.5 μm, 4.6×150 mm ID (Part AWT094269). Solvent A was 80% formate ammonium in water (w/v) 20 mM pH4.5+20% acetonitrile (v/v), and Solvent B was 20% formate ammonium in water (w/v) 20 mM pH4.5+80% ACN (v/v).

Method: Column is equilibrated with solvent A for 7 min. Gradient of 100% Solvent A to 100% solvent B in 30 min. Flow rate of 1.000 ml/min. UV detection at 254 nm. Fluorescence detection with ex. λ of 480 and em. λ of 560 nm. Purity of the compounds was assessed as the percentage surface area of the peaks.

E. Cultured Cells

MCF-7/6 cultured cells were graciously provided by Professor M. Mareel from the Ghent University (Belgium).

The B16-B16 cell line was obtained from the ATCC.

LS-174-T, the trypsinized variant of LS-180 (CL-187) colon adenocarcinoma line, was obtained from ATCC (CL 188)

F. Culture Medium and Routine Cell Culture

DMEM-F12 (Gibco) culture medium was used for all types of cultured cells. It was completed with 10% BFS (Gibco). A so-called “defined” culture medium was used for the preparation of the cell-conditioned medium.

1. Serum-containing Medium

Serum-containing medium was generally composed of a basic culture medium completed with 10% inactivated BFS. Inactivation was accomplished by incubating the serum at 56° C. for 30 min in a water bath. L-Glutamine (2 mM) was also added. Some culture media already contained a stable form of the L-glutamine equivalent, called glutamax. 100 IU/ml of penicillin and 0.1 mg/ml of streptomycin could also be added to the cultured cells to avoid bacterial contamination.

2. Defined Medium

The medium was prepared with DMEM-F12 without phenol red, and completed with the following products: 10 μg/ml human apotransferrin, 200 μg/ml BSA, 1 μg/ml insulin and 10 nM of 17-β-estradiol.

No serum was added to the medium.

3. Routine Cell Culture

All cell types were commonly cultured in sterile TC/PS flasks, maintained in a standard incubator at 37° C. (95% O ₂and 5% CO ₂). The culture medium was changed every 2-3 days.

Confluent cells were harvested for transfer into new flasks. This was done by rinsing the confluent cell layer with PBS containing 0.53 mM EDTA, completed with 0.25% trypsin. When harvested (from 5-10 min of incubation with the trypsin-containing solution), the cells were suspended in a fresh serum-containing culture medium and centrifuged at 300 g for 10 mm. The pellet was resuspended in fresh medium and distributed into new flasks.

G. Preparation of Medium Conditioned by MCF-7/6 Cancer Cells (“CM”)

MCF 7/6 cells were grown until subconfluence (not entirely continuous cell layer) in complete medium in 175 cm ²TC/PS flasks. The cells were then washed with PBS (3×), 15 ml of defined medium was added to each flask, and the cells incubated for 24 hr at standard conditions.

The preparation of the CM, concentrated 20× was as follows. The medium was recovered, cooled down to 4° C. and centrifuged at 300×g for 10 min to remove suspended cells. The medium was then concentrated 20× using an Amicon concentrator (Millipore) containing membranes with a cut off at 10 kDa (YM10-Millipore).

H. Preparation of Drug Solutions

Compounds to be tested in biological experiments (stability, uptake, c

4296105	Derivatives of doxorubicine, their preparation and use	October, 1981	Baurain et al.
5659061	Tumor protease activated prodrugs of phosphoramide mustard analogs with toxification and detoxification functionalities	August, 1997	Glazier	558/166
5962216	Tumor-activated prodrug compounds and treatment	October, 1999	Trouet et al.
20030027757	Novel molecules of the PYRIN/NBS/LRR protein family and uses thereof	February, 2003	Bertin et al.	514/12

WO/1996/005863	February, 1996			COMPOUNDS, PHARMACEUTICAL COMPOSITION AND DIAGNOSTIC DEVICE COMPRISING SAME AND THEIR USE
WO/2000/033888	June, 2000			PRODRUG COMPOUNDS AND PROCESS FOR PREPARATION THEREOF